Catalog Number - 85518-15P-2

The 85518‑15P‑2 Cogent RP C18 ABX™ HPLC Column is engineered for maximum‑resolution separations in low‑flow LC‑MS workflows, providing reliable, rugged performance across a wide pH range (2–12).

Constructed with a bulky, specially end‑capped C18 phase bonded to high‑purity silica, this column reduces secondary interactions and enhances peak shape for acidic, basic, hydrophobic, and neutral compounds, including analytes with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for demanding acid/base methods

✅ One of the most popular ABX columns for LC‑MS workflows requiring durability and resolution

150 mm Length – Maximum Resolution for LC‑MS Methods

• Highest resolving power in the 5 µm 2.1 mm ABX column range

• Maximizes peak capacity for complex mixtures

• Ideal for closely related compounds and difficult separations

• Preferred for final method optimization and validation workflows

✅ Selected when maximum separation performance is required beyond 50 mm and 100 mm columns

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for low‑flow HPLC and LC‑MS systems

• Enhances MS sensitivity with reduced solvent consumption

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires:

• Precise low‑flow pump delivery

• Low‑volume detector flow cells

⚠️ Narrow‑bore systems require stable, accurate flow control for best results

5 µm Particle – Maximum Robustness & Reproducibility

• Provides:

  • Long column lifetime

  • Excellent durability under routine use

  • Lower backpressure vs smaller particles

✅ Ideal for:

• Routine LC‑MS workflows

• Validated methods and method transfer

• Systems with pressure limitations

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phase conditions

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains structural integrity under harsh analytical environments

✅ Enables separations beyond standard C18 column limitations

Why Choose RP C18 ABX™ Columns (5 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for stability‑indicating and aggressive analytical methods

Maximum Durability & Method Transferability

• Designed for long‑term, reproducible performance

• Supports standardization across labs and systems

Versatile Analytical Performance

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

✅ Preferred for routine, validated LC‑MS workflows requiring consistency

LC‑MS Workflow Advantages

• Narrow‑bore 2.1 mm format:

  • Improves detection sensitivity

  • Reduces solvent usage

• 150 mm length:

  • Maximizes resolution and peak capacity

  • Enhances separation of complex mixtures

✅ Ideal for high‑resolution LC‑MS workflows with demanding separations

System & Sample Considerations

• Designed for low‑flow LC‑MS and analytical HPLC systems

• Requires:

  • Accurate pump control at low flow rates

  • Low‑volume detection systems

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column clogging

• Maintain long‑term performance and reproducibility

Typical Applications

• LC‑MS and LC‑MS/MS analysis

• Acidic and basic compound separations

• Pharmaceutical QC and validation

• Stability‑indicating methods

• Complex mixture separations

• High‑resolution analytical workflows

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow systems (~0.2–0.3 mL/min)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for maximum‑resolution LC‑MS workflows

• Ensure system capability for low‑flow stability and precision